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    劉晨臨,王秀良,林學政.南極紅藻Iridaea cordataCurdiea racovitzae轉錄組分析及其極端光環境適應相關基因的挖掘[J].海洋學報,2020,42(10):110-120
    南極紅藻Iridaea cordataCurdiea racovitzae轉錄組分析及其極端光環境適應相關基因的挖掘
    De nova transcriptome analysis and mining extreme light environments acclimation responding genes of Antarctic seaweed Iridaea cordata (Gigartinales, Rhodophyta) and Curdiea racovitzae (Gracilariaceae, Rhodophyta)
    投稿時間:2019-07-02  修訂日期:2019-11-18
    DOI:10.3969/j.issn.0253-4193.2020.10.011
    中文關鍵詞:  南極紅藻  轉錄組  極端光環境適應  Iridaea cordata  Curdiea racovitzae
    英文關鍵詞:Antarctic red algae  transcriptome  extreme light environmental acclimation  Iridaea cordata  Curdiea racovitzae
    基金項目:國家自然科學基金(41276203);國家南極觀測監測網運維與管理項目(JDKC0518013)。
    作者單位
    劉晨臨 自然資源部第一海洋研究所,山東 青島 266061
    青島海洋科學與技術試點國家實驗室 海洋生物學與生物技術實驗室,山東 青島 266237 
    王秀良 中國科學院海洋研究所,山東 青島 266071
    青島海洋科學與技術試點國家實驗室 海洋生物學與生物技術實驗室,山東 青島 266237 
    林學政 自然資源部第一海洋研究所,山東 青島 266061 
    摘要點擊次數: 1188
    全文下載次數: 4216
    中文摘要:
          南極紅藻具有重要的生態學功能和開發利用價值。南極極端環境賦予了其獨特的生理耐受機制,也是發現新基因和代謝途徑的理想材料。我們測序分析了南極產膠紅藻Iridaea cordata (Turner) Bory和Curdiea racovitzae Hariot的轉錄組序列,并與其常溫近緣種進行了比較,同時挖掘了其與光限制和強紫外線輻射等光環境適應相關的基因。I. cordataC. racovitzae的轉錄組序列分別拼接成了14055條和12006條非冗余基因,平均長度分別為1473 bp和1448 bp。在I. cordata轉錄組中發現多條與綠藻基因同源的捕光復合物LHC基因Lhca2、Lhca6Lhcb,并且在兩種南極紅藻中都各發現了1條編碼結合巖藻黃質和Chl a/c蛋白的Lhcf基因,目前尚未在其他紅藻中發現這類基因。光裂解酶修復紫外線誘導DNA損傷,在I. cordata的轉錄組序列中發現了6-4光裂解酶,光裂解酶CPD I和CPD II基因,而C. racovitzae轉錄組序列中僅找到了光裂解酶CPD II基因。盡管南極紅藻中這些特有基因的功能有待進一步的驗證,但是本文為后續研究紅藻的南極極端光環境適應機制提供了方向。
    英文摘要:
          Antarctic red algae play important roles in the coastal ecosystems and industrial applications. Meanwhile, their unique physiological acclimation mechanisms to the extreme environments endow them to be ideal organisms for discovering new genes and new metabolic pathways. In this study, we sequenced the transcriptomes of Antarctic red algae Iridaea cordata (Turner) Bory and Curdiea racovitzae Hariot, and compared with their moderate temperature close relatives. The transcriptome sequences of I. cordata and C. racovitzae were assembled into 14055 and 12006 Unigenes, with an average length of 1473 bp and 1448 bp, respectively. The Lhca2, Lhca6 and Lhcb genes homologous to the green algae genes were found in I. cordata transcriptome while not in other red algae. Lhcf gene encoding fucoxanthine and Chl a/c binding protein presenting in brown algae and diatoms were identified in both I. cordata and C. racovitzae. Photolyase repairs UV-induced DNA damages. 6-4 photolyase, CPD I and CPD II genes were identified in the transcriptome of I. cordata, while only CPD II gene was found in the transcriptome of C. racovitzae. Although the functions of those specific genes in Antarctic red algae are expected further investigation, our study provides a foundation for the following researches on the acclimation mechanisms of seaweeds to the extreme light environments in Antarctica.
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