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    一株分離自膠州灣的裸甲藻形態相似種的分子系統學研究
    Molecular phylogenetic analysis of a Gymnodinium-like species isolated from Jiaozhou Bay, P.R. China
    投稿時間:2010-02-05  修訂日期:2010-06-07
    DOI:
    中文關鍵詞:  核糖體DNA(rDNA)  轉錄間隔區(internal transcribed spacer, ITS)  裸甲藻(Gymnodinium)  共生藻(Symbiodinium)
    英文關鍵詞:rDNA  ITS  Gymnodinium  Symbiodinium
    基金項目:國家自然科學基金項目(面上項目,重點項目,重大項目)
    作者單位郵編
    朱霞 中國海洋大學 266100
    甄毓 中國海洋大學 266100
    于志剛 中國海洋大學 
    摘要點擊次數: 3971
    全文下載次數: 601
    中文摘要:
           本研究對一株分離自膠州灣的裸甲藻形態相似種(編號為Gymnodinium sp. ZX)進行分子水平的分類鑒定。提取基因組DNA后擴增核糖體小亞基和轉錄間隔區序列,經純化、克隆并測序。將獲得的序列分別進行Blastn同源性分析,并下載相關序列構建系統進化樹。結果表明,該藻與共生藻(Symbiodinium)親緣關系較近,而與裸甲藻(Gymnodinium)等其它甲藻親緣關系較遠,因此初步鑒定該藻為一種共生藻。用核糖體小亞基和轉錄間隔區序列分別對共生藻屬構建系統進化樹,分析各類群之間的關系,初步鑒定本藻屬于共生藻屬E類群。該藻分離自膠州灣,在f/2培養液中生長良好,表明膠州灣可能存在營自由生活的共生藻。
    英文摘要:
          In this study, a strain of microalgae that was isolated from Jiaozhou Bay and primarily identified as Gymnodinium-like based on the morphological features (named as Gymnodinium sp. ZX) was further distinguished with molecular phylogenetic methods. After the processes of DNA extraction, amplification and purification, the nuclear-encoded small subunit ribosomal DNA (SSU rDNA) and internal transcribed spacer (ITS) regions were cloned and sequenced. Sequences homologous to SSU rDNA and ITS regions of Gymnodinium sp. ZX were searched with Blastn from the GenBank. The phylogenetic trees were reconstructed with the neighbor-joining (NJ) method. The results showed that Gymnodinium sp. ZX had a close genetic relationship with Symbiodinium rather than Gymnodinium. Therefore, Gymnodinium sp. ZX was judged as a strain of Symbiodinium sp.. Then the phylogenetic trees were reconstructed based on the SSU rDNA and ITS sequences of genus Symbiodinium respectively, the relationship among the various clades was analyzed, and Gymnodinium sp. ZX belonged to clade E. The Gymnodinium sp. ZX was isolated from Jiaozhou Bay, and grew well in the f/2 medium, therefore, it was inferred that the free-living species of genus Symbiodinium probably existed in Jiaozhou Bay.
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